實驗名稱：微流(liu)控芯片中操控液(ye)滴充(chong)電分選(xuan)實驗

　　研究方向：微流控芯片液滴操(cao)控與分(fen)選(xuan)技術

　　實驗內容：通(tong)(tong)過靜電(dian)(dian)(dian)(dian)誘導機制對液(ye)滴(di)(di)進行(xing)充電(dian)(dian)(dian)(dian)，利用(yong)非均勻電(dian)(dian)(dian)(dian)場實現(xian)帶電(dian)(dian)(dian)(dian)液(ye)滴(di)(di)的(de)偏轉分選。充電(dian)(dian)(dian)(dian)信號(hao)由信號(hao)發生(sheng)(sheng)器(qi)產生(sheng)(sheng)，經ATA-2161高(gao)壓放大器(qi)放大后施加至充電(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)極，使得(de)液(ye)滴(di)(di)表面積累電(dian)(dian)(dian)(dian)荷。帶電(dian)(dian)(dian)(dian)液(ye)滴(di)(di)在偏轉電(dian)(dian)(dian)(dian)極產生(sheng)(sheng)的(de)電(dian)(dian)(dian)(dian)場作用(yong)下(xia)定(ding)向偏轉至目標收(shou)集通(tong)(tong)道。實驗(yan)(yan)分析了(le)液(ye)滴(di)(di)生(sheng)(sheng)成頻(pin)率、充電(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)壓、脈沖寬度(du)等因素(su)對分選精度(du)的(de)影響，驗(yan)(yan)證了(le)系統在液(ye)滴(di)(di)分選中(zhong)高(gao)活性(xing)和(he)高(gao)效率的(de)性(xing)能(neng)。。

　　測試設備：信號(hao)發(fa)生器、ATA-2161高(gao)(gao)壓放大器、高(gao)(gao)壓直流電源(yuan)、高(gao)(gao)速相機、微流控芯片等。

　　實驗過程：

　　圖1：微流控芯片中操控液滴(di)充電分選實驗裝置圖

　　圖2：微流控(kong)芯片中操控(kong)液滴充電分(fen)選實(shi)驗

　　本(ben)實驗利用(yong)信(xin)號發(fa)生(sheng)器、ATA-2161高(gao)(gao)(gao)壓(ya)(ya)放大器、高(gao)(gao)(gao)壓(ya)(ya)直流(liu)電(dian)(dian)(dian)(dian)(dian)源(yuan)和高(gao)(gao)(gao)速相機(ji)搭建(jian)了液(ye)滴(di)充電(dian)(dian)(dian)(dian)(dian)分選(xuan)測試平(ping)臺。信(xin)號發(fa)生(sheng)器輸(shu)出(chu)充電(dian)(dian)(dian)(dian)(dian)脈(mo)沖信(xin)號，通過(guo)ATA-2161功(gong)率放大器放大后施加(jia)至充電(dian)(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)(dian)極，實現液(ye)滴(di)的(de)(de)電(dian)(dian)(dian)(dian)(dian)荷(he)積(ji)累。高(gao)(gao)(gao)壓(ya)(ya)直流(liu)電(dian)(dian)(dian)(dian)(dian)源(yuan)產生(sheng)偏(pian)轉(zhuan)電(dian)(dian)(dian)(dian)(dian)場，引(yin)導帶電(dian)(dian)(dian)(dian)(dian)液(ye)滴(di)定向偏(pian)轉(zhuan)至目(mu)標通道。高(gao)(gao)(gao)速相機(ji)監(jian)控液(ye)滴(di)生(sheng)成與(yu)分選(xuan)過(guo)程，熒(ying)光檢測系(xi)統(tong)用(yong)于評估分選(xuan)精度(du)與(yu)細胞(bao)活性(xing)，驗證系(xi)統(tong)的(de)(de)高(gao)(gao)(gao)效率和高(gao)(gao)(gao)活性(xing)。

　　實驗結果：

圖(tu)：單個液(ye)滴(di)充電分選過(guo)程圖(tu)

　　圖：不同充電時長(chang)液(ye)滴偏轉結果(guo)圖

　　實驗結(jie)果表(biao)明，基于ATA-2161高(gao)壓放(fang)大(da)器的(de)靜電誘導(dao)分(fen)選系(xi)統通過時(shi)(shi)序調控與(yu)電場(chang)優(you)化(hua)(hua)實現了(le)高(gao)精度(du)液(ye)滴(di)操(cao)控，充(chong)電脈沖寬度(du)為液(ye)滴(di)生(sheng)成時(shi)(shi)間（0.625ms）的(de)整數倍（n=1-10），調控單次觸發(fa)分(fen)選液(ye)滴(di)數（4/6/8/10）。該系(xi)統通過時(shi)(shi)序標(biao)定、參數匹(pi)配及結(jie)構(gou)優(you)化(hua)(hua)，實現了(le)亞毫秒級分(fen)選控制能力，為高(gao)活性細胞與(yu)微球(qiu)的(de)精準(zhun)操(cao)控提供了(le)可靠(kao)解決方案。

　　功率放大(da)器推薦：ATA-2161

　　圖：ATA-2161高壓放大器指標參數

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